Journal: Chemistry & biology

Article Title: Biosynthetic pathway and structure of aeruginosides 126A and 126B, cyanobacterial peptides bearing a 2-carboxy-6-hydroxyoctahydroindole moiety

doi: 10.1016/j.chembiol.2007.04.006

Figure Lengend Snippet: Deduced Functions of ORFs in the Aeruginoside Biosynthetic Gene Cluster

Article Snippet: Protein Amino Acids Deduced Function Sequence Similarity Identity/SimilarityAccession Number (AA length) AerA 1416 PKS (A KR ACP) BarE ( Lyngbya majuscula ) 44%/64% (587) AAN32979 AerB 1598 NRPS (C A T E) NosC ( Nostoc sp. GSV224) 54%/71% (944) AAF17280 AerC 736 Oxygenase Putative diaminopimelate decarboxylase ( Burkholderia mallei 10229) 30%/49% (707)ZP00431845 AerD 202 Decarboxylase BacA ( Bacillus amyloliquefaciens AAM90573 ) 39%/58% (191) AAM90573 AerE 213 Unknown BacB ( Bacillus subtilis ) 26%/45% (208) AAM90569 AerF 264 Reductase BacC ( Bacillus subtilis ) 36%/55% (256)AA65204 AerG 1622 NRPS (C A T C T) McyB ( Microcystis aeruginosa ) 47%/64% (927) AAE09608 AerH 308 Dioxygenase Thymine dioxygenase ( Rhodotorula glutinis ) 30%/50% (517) AAU12179 AerI 418 Glycosyltransferase Spore coat protein SA ( Bacillus subtilis ) 26%/46% (336) P46915 ORF1 248 Circadian clock protein KaiA ( Synechococcus elongatus ) 31%/51% (227)1R8JA ORF2 126 Unknown no similarity ORF3 288 Unknown no similarity ORF4 229 Unknown no similarity ORF5 170 Unknown Putative reverse transcriptase ( Nostoc sp. PCC7120) 70%/83% (157) BAB77479 ORF6 428 Unknown Retron-type reverse transcriptase ( Anabaena variabilis ATCC29413) 58%/74% (415) ABA24728 ORF7 386 Oxidoreductase Putative aldo/keto reductase ( Tricodesmium erythraeum IMS101) 70%/84% (383)ZP00328942 ORF8 339 Sulfotransferase Putative sulfotransferase ( Shewanella sp. PV-4) 22%/39% (228)EAP04083 ORF9 664 ABC-type transporter NcpC ( Nostoc sp. ATCC 53789) 57%/75% /663) AAO23332 ORF10 257 Succinate dehydrogenase Succinate dehydrogenase ( Sulfolobus tokodaii ) 33%/53% (238) BAB40685 Open in a separate window Deduced Functions of ORFs in the Aeruginoside Biosynthetic Gene Cluster.

Techniques: Sequencing, Reverse Transcription

Journal: Cell reports

Article Title: Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in MYC-driven medulloblastoma.

doi: 10.1016/j.celrep.2021.109013

Figure Lengend Snippet: Figure 3. Low-dose THZ1 silences MYC-activated genes (A) Log IC50 determination of THZ1 and THZ2 on group 3 MB cell lines and non-MYC-amplified cell lines. n = 3. Mean ± SEM. Color scale indicates low (green) to high (red) concentrations. (B) Representative images of methylcellulose assay of D458 or D425 cells treated with DMSO or 2 (IC20), 7 (IC30), or 10 nM (IC50) THZ1. n = 3. Scatterplot of colonies/well, mean ± SD. Statistical analysis, one-way ANOVA. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05. (C) Immunoblot of proteins from D458, D425, ONS76-RFP, and ONS76-MYC treated with IC50 of THZ1 or THZ2 Average density below blot, n = 3. See also Figures S3D and S3E for quantification (CPARP, cleaved PARP). (D) RNA-seq gene expression heatmap of THZ1 IC50 treated D458 and untreated cells. Calculated Z score scale is shown.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal Phospho-Rpb1 CTD (Ser5) Cell Signaling Cat#13523;RRID:AB_2798246 Rabbit monoclonal Phospho-Rpb1 CTD (Ser2) Cell Signaling Cat#13499;RRID:AB_2798238 Mouse monoclonal CDK7 Cell Signaling Cat#2916;RRID:AB_2077142 Rabbit monoclonal CDK9 Cell Signaling Cat#2316;RRID:AB_2291505 Rabbit polyclonal PARP/cleaved PARP Cell Signaling Cat#9542;RRID:AB_2160739 Rabbit monoclonal cMYC Cell Signaling Cat#5605;RRID:AB_1903938 Rabbit polyclonal RNA Pol II Bethyl Laboratories Cat#A300-653A; RRID:AB_519334 Anti-Mouse IgG-HRP linked Cell Signaling Cat#7076;RRID:AB_330924 Anti-Rabbit IgG-HRP linked Cell Signaling Cat#7074;RRID:AB_2099233 Mouse monoclonal b-Actin-HRP linked Cell Signaling Cat#12262;RRID:AB_2566811 Rabbit monoclonal Phospho-Histone H2A.X (Ser139) Cell Signaling Cat#9718;RRID:AB_2118009 Rabbit polyclonal RPA70/RPA1 Cell Signaling Cat#2267;RRID:AB_2180506 Rabbit monoclonal Ki67 Thermo Fisher Scientific Cat#RM-9106; RRID:AB_2335745 Mouse monoclonal RAD51 Novus Biologicals Cat#NB100-148; RRID:AB_10002131 Rabbit polyclonal Cleaved Caspase 3 Cell Signaling Cat#9661:RRID:AB_2341188 Rabbit monoclonal RNA Polymerase II CTD abCAM Cat#ab817; RRID:AB_306327 Rabbit monoclonal cMYC Cell signaling Cat#13987;RRID:AB_2631168 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 488 Conjugated Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 555 Conjugated Thermo Fisher Scientific Cat# A-31572; RRID:AB_162543 Biological samples Normal Cerebellum paraffin blocks Dr. Nicholas Foreman lab N/A Patient embedded tumor paraffin blocks Dr. Nicholas Foreman lab N/A Chemicals, peptides, and recombinant proteins THZ1 Cayman Chemical Cat#9002215 THZ2 MedChem Express Cat#HY-12280/CS-3245 YKL-5-124 Selleckchem Cat#S8863 CT7001 MedChem Express Cat#HY-103712A Nitrotetrazolium blue chloride Sigma Aldrich Cat# N6876-50MG Critical commercial assays Guava Nexin Assay Millipore Cat#4500-0450 CellTiter 96 Aqueous One Solution Cell Proliferation Promega Cat#G3580 RNeasy Mini Kit QIAGEN Cat#74104 DNeasy Blood and Tissue Kit QIAGEN Cat#69504 High Capacity Reverse Transcription Kit Thermo Fisher Scientific Cat#4368814 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat#4400040 Venor GeM Mycoplasma Detection Kit Sigma Aldrich Cat#MP0025-1kt Qubit dsDNA High Sensitivity Assay Thermo Fisher Scientific Cat#Q32851 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000008 (Continued on next page) e1 Cell Reports 35, 109013, April 27, 2021

Techniques: Methylcellulose Assay, Western Blot, RNA Sequencing, Gene Expression

Journal: Cell reports

Article Title: Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in MYC-driven medulloblastoma.

doi: 10.1016/j.celrep.2021.109013

Figure Lengend Snippet: Figure 5. CDK7 inhibition abrogates RNA Pol II and MYC promoter association (A) ChIP sequencing with RNA Pol II and MYC antibody performed on D458 cells treated with DMSO versus 10 nM THZ1. Heatmap of normalized RNA Pol II or MYC at the TSS. (Bottom) average read density of RNA Pol II or MYC ChIP sequencing at the TSS. DMSO (blue) or 10 nM THZ1 (yellow). (B) GO functional categories for RNA Pol II ChIP cluster 1 genes affected by THZ1 treatment. Enrichment scores are shown as -log10(Pval). (C) GO functional categories for MYC ChIP cluster 1 genes affected by THZ1 treatment. Enrichment scores are shown as -log10(Pval). (D) Box-and-whisker plots ± min and max of the total number of reads within 200 bp of the TSS for the top genes within the DNA Repair ontology of cluster 1 for RNA Pol II ChIP and MYC ChIP. MYC ChIP read numbers for top DNA repair genes (right). Statistical analysis, two-tailed, unpaired t test. ****p < 0.0001; **p < 0.01. Color scale indicates high (red) to low (white) reads. (E) Top genes within the mRNA processing ontology of cluster 2 for RNA Pol II and MYC ChIPs. Statistical analysis, two-tailed, unpaired t test. ns, not significant. See also Figure S5.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal Phospho-Rpb1 CTD (Ser5) Cell Signaling Cat#13523;RRID:AB_2798246 Rabbit monoclonal Phospho-Rpb1 CTD (Ser2) Cell Signaling Cat#13499;RRID:AB_2798238 Mouse monoclonal CDK7 Cell Signaling Cat#2916;RRID:AB_2077142 Rabbit monoclonal CDK9 Cell Signaling Cat#2316;RRID:AB_2291505 Rabbit polyclonal PARP/cleaved PARP Cell Signaling Cat#9542;RRID:AB_2160739 Rabbit monoclonal cMYC Cell Signaling Cat#5605;RRID:AB_1903938 Rabbit polyclonal RNA Pol II Bethyl Laboratories Cat#A300-653A; RRID:AB_519334 Anti-Mouse IgG-HRP linked Cell Signaling Cat#7076;RRID:AB_330924 Anti-Rabbit IgG-HRP linked Cell Signaling Cat#7074;RRID:AB_2099233 Mouse monoclonal b-Actin-HRP linked Cell Signaling Cat#12262;RRID:AB_2566811 Rabbit monoclonal Phospho-Histone H2A.X (Ser139) Cell Signaling Cat#9718;RRID:AB_2118009 Rabbit polyclonal RPA70/RPA1 Cell Signaling Cat#2267;RRID:AB_2180506 Rabbit monoclonal Ki67 Thermo Fisher Scientific Cat#RM-9106; RRID:AB_2335745 Mouse monoclonal RAD51 Novus Biologicals Cat#NB100-148; RRID:AB_10002131 Rabbit polyclonal Cleaved Caspase 3 Cell Signaling Cat#9661:RRID:AB_2341188 Rabbit monoclonal RNA Polymerase II CTD abCAM Cat#ab817; RRID:AB_306327 Rabbit monoclonal cMYC Cell signaling Cat#13987;RRID:AB_2631168 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 488 Conjugated Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 555 Conjugated Thermo Fisher Scientific Cat# A-31572; RRID:AB_162543 Biological samples Normal Cerebellum paraffin blocks Dr. Nicholas Foreman lab N/A Patient embedded tumor paraffin blocks Dr. Nicholas Foreman lab N/A Chemicals, peptides, and recombinant proteins THZ1 Cayman Chemical Cat#9002215 THZ2 MedChem Express Cat#HY-12280/CS-3245 YKL-5-124 Selleckchem Cat#S8863 CT7001 MedChem Express Cat#HY-103712A Nitrotetrazolium blue chloride Sigma Aldrich Cat# N6876-50MG Critical commercial assays Guava Nexin Assay Millipore Cat#4500-0450 CellTiter 96 Aqueous One Solution Cell Proliferation Promega Cat#G3580 RNeasy Mini Kit QIAGEN Cat#74104 DNeasy Blood and Tissue Kit QIAGEN Cat#69504 High Capacity Reverse Transcription Kit Thermo Fisher Scientific Cat#4368814 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat#4400040 Venor GeM Mycoplasma Detection Kit Sigma Aldrich Cat#MP0025-1kt Qubit dsDNA High Sensitivity Assay Thermo Fisher Scientific Cat#Q32851 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000008 (Continued on next page) e1 Cell Reports 35, 109013, April 27, 2021

Techniques: Inhibition, ChIP-sequencing, Functional Assay, Whisker Assay, Two Tailed Test

Journal: Cell reports

Article Title: Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in MYC-driven medulloblastoma.

doi: 10.1016/j.celrep.2021.109013

Figure Lengend Snippet: Figure 6. CDK7 inhibition minimizes DNA damage response enhancing susceptibility to IR (A) GSEA from THZ1 D458 treatment RNA-seq. Kauffman DNA repair, KEGG homologous recombination. FDR q value = 0.0. (B) Volcano plot of Kauffman DNA repair (green), KEGG homologous recombination (yellow), and MYC targets (blue) from THZ1-treated D458 cells. (C) Individual ChIP gene tracks of RNA Pol II, MYC signals, and RNA transcript profile for DMSO and THZ1 10-nM D458 treatments. Shown are the y axis signal density (rpm/bp) of BRCA2 promoter and RAD51C promoter.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal Phospho-Rpb1 CTD (Ser5) Cell Signaling Cat#13523;RRID:AB_2798246 Rabbit monoclonal Phospho-Rpb1 CTD (Ser2) Cell Signaling Cat#13499;RRID:AB_2798238 Mouse monoclonal CDK7 Cell Signaling Cat#2916;RRID:AB_2077142 Rabbit monoclonal CDK9 Cell Signaling Cat#2316;RRID:AB_2291505 Rabbit polyclonal PARP/cleaved PARP Cell Signaling Cat#9542;RRID:AB_2160739 Rabbit monoclonal cMYC Cell Signaling Cat#5605;RRID:AB_1903938 Rabbit polyclonal RNA Pol II Bethyl Laboratories Cat#A300-653A; RRID:AB_519334 Anti-Mouse IgG-HRP linked Cell Signaling Cat#7076;RRID:AB_330924 Anti-Rabbit IgG-HRP linked Cell Signaling Cat#7074;RRID:AB_2099233 Mouse monoclonal b-Actin-HRP linked Cell Signaling Cat#12262;RRID:AB_2566811 Rabbit monoclonal Phospho-Histone H2A.X (Ser139) Cell Signaling Cat#9718;RRID:AB_2118009 Rabbit polyclonal RPA70/RPA1 Cell Signaling Cat#2267;RRID:AB_2180506 Rabbit monoclonal Ki67 Thermo Fisher Scientific Cat#RM-9106; RRID:AB_2335745 Mouse monoclonal RAD51 Novus Biologicals Cat#NB100-148; RRID:AB_10002131 Rabbit polyclonal Cleaved Caspase 3 Cell Signaling Cat#9661:RRID:AB_2341188 Rabbit monoclonal RNA Polymerase II CTD abCAM Cat#ab817; RRID:AB_306327 Rabbit monoclonal cMYC Cell signaling Cat#13987;RRID:AB_2631168 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 488 Conjugated Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 555 Conjugated Thermo Fisher Scientific Cat# A-31572; RRID:AB_162543 Biological samples Normal Cerebellum paraffin blocks Dr. Nicholas Foreman lab N/A Patient embedded tumor paraffin blocks Dr. Nicholas Foreman lab N/A Chemicals, peptides, and recombinant proteins THZ1 Cayman Chemical Cat#9002215 THZ2 MedChem Express Cat#HY-12280/CS-3245 YKL-5-124 Selleckchem Cat#S8863 CT7001 MedChem Express Cat#HY-103712A Nitrotetrazolium blue chloride Sigma Aldrich Cat# N6876-50MG Critical commercial assays Guava Nexin Assay Millipore Cat#4500-0450 CellTiter 96 Aqueous One Solution Cell Proliferation Promega Cat#G3580 RNeasy Mini Kit QIAGEN Cat#74104 DNeasy Blood and Tissue Kit QIAGEN Cat#69504 High Capacity Reverse Transcription Kit Thermo Fisher Scientific Cat#4368814 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat#4400040 Venor GeM Mycoplasma Detection Kit Sigma Aldrich Cat#MP0025-1kt Qubit dsDNA High Sensitivity Assay Thermo Fisher Scientific Cat#Q32851 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000008 (Continued on next page) e1 Cell Reports 35, 109013, April 27, 2021

Techniques: Inhibition, RNA Sequencing, Homologous Recombination

Journal: Cell reports

Article Title: Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in MYC-driven medulloblastoma.

doi: 10.1016/j.celrep.2021.109013

Figure Lengend Snippet: Figure 7. In vivo IR with CDK7 inhibition (A) Representative bioluminescence images of xenograft D458 vehicle or THZ2 15 mg/kg treated with 1.5 Gy over 5 days starting at day 15 post-injection. Color scales indicate bioluminescence radiance in photons/sec/cm2/steradian. (B) Representative MRI of vehicle + IR and THZ2 + IR treated D458 xenograft mice at 23 and 49 days. White arrows indicate tumor. (C) Box-and-whisker plot ± min and max of mean total radiance in photons/sec/cm2/steradian. Statistical analysis, two-way ANOVA, ****p < 0.0001. (D) Kaplan-Meier survival curve of D458 xenograft mice treated with vehicle (n = 9) or THZ2 (n = 10) and 1.5 Gy for 5 days (violet box). THZ2 treatment period for 25 days, shaded box. Statistical analysis, log-rank (Mantel-Cox) test, *p < 0.05. (E) MYC gene amplification in group 3 MB drives highly active transcription of DNA repair, proliferation, and self-renewal genes shielding cells against IR and sustaining growth. Treatment with THZ1/THZ2 blocks RNA Pol II association at the MYC promoter, limiting MYC transcription, and blocks RNA Pol II and MYC association with select gene promoters, removing MB tumor cells’ defense mechanisms and leading to cell death. Image created with BioRender.com.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal Phospho-Rpb1 CTD (Ser5) Cell Signaling Cat#13523;RRID:AB_2798246 Rabbit monoclonal Phospho-Rpb1 CTD (Ser2) Cell Signaling Cat#13499;RRID:AB_2798238 Mouse monoclonal CDK7 Cell Signaling Cat#2916;RRID:AB_2077142 Rabbit monoclonal CDK9 Cell Signaling Cat#2316;RRID:AB_2291505 Rabbit polyclonal PARP/cleaved PARP Cell Signaling Cat#9542;RRID:AB_2160739 Rabbit monoclonal cMYC Cell Signaling Cat#5605;RRID:AB_1903938 Rabbit polyclonal RNA Pol II Bethyl Laboratories Cat#A300-653A; RRID:AB_519334 Anti-Mouse IgG-HRP linked Cell Signaling Cat#7076;RRID:AB_330924 Anti-Rabbit IgG-HRP linked Cell Signaling Cat#7074;RRID:AB_2099233 Mouse monoclonal b-Actin-HRP linked Cell Signaling Cat#12262;RRID:AB_2566811 Rabbit monoclonal Phospho-Histone H2A.X (Ser139) Cell Signaling Cat#9718;RRID:AB_2118009 Rabbit polyclonal RPA70/RPA1 Cell Signaling Cat#2267;RRID:AB_2180506 Rabbit monoclonal Ki67 Thermo Fisher Scientific Cat#RM-9106; RRID:AB_2335745 Mouse monoclonal RAD51 Novus Biologicals Cat#NB100-148; RRID:AB_10002131 Rabbit polyclonal Cleaved Caspase 3 Cell Signaling Cat#9661:RRID:AB_2341188 Rabbit monoclonal RNA Polymerase II CTD abCAM Cat#ab817; RRID:AB_306327 Rabbit monoclonal cMYC Cell signaling Cat#13987;RRID:AB_2631168 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 488 Conjugated Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Polyclonal Antibody, Alexa Fluor 555 Conjugated Thermo Fisher Scientific Cat# A-31572; RRID:AB_162543 Biological samples Normal Cerebellum paraffin blocks Dr. Nicholas Foreman lab N/A Patient embedded tumor paraffin blocks Dr. Nicholas Foreman lab N/A Chemicals, peptides, and recombinant proteins THZ1 Cayman Chemical Cat#9002215 THZ2 MedChem Express Cat#HY-12280/CS-3245 YKL-5-124 Selleckchem Cat#S8863 CT7001 MedChem Express Cat#HY-103712A Nitrotetrazolium blue chloride Sigma Aldrich Cat# N6876-50MG Critical commercial assays Guava Nexin Assay Millipore Cat#4500-0450 CellTiter 96 Aqueous One Solution Cell Proliferation Promega Cat#G3580 RNeasy Mini Kit QIAGEN Cat#74104 DNeasy Blood and Tissue Kit QIAGEN Cat#69504 High Capacity Reverse Transcription Kit Thermo Fisher Scientific Cat#4368814 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat#4400040 Venor GeM Mycoplasma Detection Kit Sigma Aldrich Cat#MP0025-1kt Qubit dsDNA High Sensitivity Assay Thermo Fisher Scientific Cat#Q32851 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000008 (Continued on next page) e1 Cell Reports 35, 109013, April 27, 2021

Techniques: In Vivo, Inhibition, Injection, Whisker Assay

Journal: Frontiers in Plant Science

Article Title: A MADS-box gene-induced early flowering pear ( Pyrus communis L.) for accelerated pear breeding

doi: 10.3389/fpls.2023.1235963

Figure Lengend Snippet: Early flowering apple. (A) Flower development on shoots grown in vitro . (B) Early flowering and spindly stems in 35S:BpMADS4 lines grown in the glasshouse. WT indicates the wild-type control. (C) Gel electrophoresis of amplified BpMADS4 DNA fragments. Asterisks denote lines that flowered within 6 months after establishment in the glasshouse. (D) Normal flower development. (E) Fruit set after pollination.

Article Snippet: The BpMADS4 full-length coding sequence ( ) (Genbank accession X99654, nucleotides 83-865) with added BamHI and XbaI restriction enzyme sites was synthesized (GenScript, https://www.genscript.com/ ) and cloned into pSAK778, positioning it between the CaMV 35S promoter and OCS terminator.

Techniques: In Vitro, Control, Nucleic Acid Electrophoresis, Amplification

Journal: Frontiers in Plant Science

Article Title: A MADS-box gene-induced early flowering pear ( Pyrus communis L.) for accelerated pear breeding

doi: 10.3389/fpls.2023.1235963

Figure Lengend Snippet: Early flowering pear. (A) Flower (arrow) developing in vitro . (B) Gel electrophoresis of amplified BpMADS4 DNA fragments in kanamycin-resistant and wild-type (WT) control plants. Black asterisks denote lines that flowered 6–18 months after establishment in the glasshouse. Red asterisks indicate lines confirmed to give rise to early flowering progeny. C, (D) Appearance of an early flowering 35S:BpMADS4 plant (C) with terminal flowers ( D , arrows). E, (F) Appearance of a WT control (E) with vegetative shoot tips ( F , arrowheads).

Article Snippet: The BpMADS4 full-length coding sequence ( ) (Genbank accession X99654, nucleotides 83-865) with added BamHI and XbaI restriction enzyme sites was synthesized (GenScript, https://www.genscript.com/ ) and cloned into pSAK778, positioning it between the CaMV 35S promoter and OCS terminator.

Techniques: In Vitro, Nucleic Acid Electrophoresis, Amplification, Control

Journal: Frontiers in Plant Science

Article Title: A MADS-box gene-induced early flowering pear ( Pyrus communis L.) for accelerated pear breeding

doi: 10.3389/fpls.2023.1235963

Figure Lengend Snippet: Appearance of first-generation hybrids. (A) Non-early flowering (left) and early flowering (right) seedlings 4 months after germination. (B) Relative BpMADS4 expression normalized to ACTIN ( ACT ) and presented as mean ± SE of three biological replicates in early flowering highly floral (HF), moderately floral (MF) and non-flowering (NF) pear seedlings. WT, wild type.

Article Snippet: The BpMADS4 full-length coding sequence ( ) (Genbank accession X99654, nucleotides 83-865) with added BamHI and XbaI restriction enzyme sites was synthesized (GenScript, https://www.genscript.com/ ) and cloned into pSAK778, positioning it between the CaMV 35S promoter and OCS terminator.

Techniques: Expressing

Journal: Frontiers in Plant Science

Article Title: A MADS-box gene-induced early flowering pear ( Pyrus communis L.) for accelerated pear breeding

doi: 10.3389/fpls.2023.1235963

Figure Lengend Snippet: A schematic diagram representing the strategies for fast breeding and improvement of pear using early flowering BpMADS4 lines (EF). Top, Fast breeding cycles to introduce a high value trait (HVT). Half of the progeny is transgenic (EF), with half of those inheriting the HVT (EF/HVT). Alternatively, HVTs are introduced by new breeding technologies (NBT), e.g. gene editing (bottom). After a desired number of breeding cycles, non-transgenic (not EF) improved HVT lines are selected. Different fruit colors indicate HVTs.

Article Snippet: The BpMADS4 full-length coding sequence ( ) (Genbank accession X99654, nucleotides 83-865) with added BamHI and XbaI restriction enzyme sites was synthesized (GenScript, https://www.genscript.com/ ) and cloned into pSAK778, positioning it between the CaMV 35S promoter and OCS terminator.

Techniques: Introduce, Transgenic Assay